Mouse monoclonal antibody raised against partial recombinant Bcar1.
Immunogen:
Recombinant protein corresponding to C-terminus of rat Bcar1.
Host:
Mouse
Reactivity:
Chicken, Human, Mouse, Rat
Specificity:
This rat sequence is highly conserved in human, mouse, and chicken p130 Cas.
Form:
Liquid
Isotype:
IgG1
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
Recommend Usage:
ELISA (1:2000)Immunocytochemistry (1:250)Immunoprecipitation (5-10 ul)Western Blot (1:1000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Subsets of the major tyrosine phosphorylation sites in Crk-associated substrate (CAS) are sufficient to promote cell migration.Shin NY, Dise RS, Schneider-Mergener J, Ritchie MD, Kilkenny DM, Hanks SK.J Biol Chem. 2004 Sep 10;279(37):38331-7. Epub 2004 Jul 6.
2.
Phosphorylation-dependent cleavage of p130cas in apoptotic rat-1 cells.Hoon Kim D, Jeon Choi S, Kook S, Kim W, Keun Song W.Biochem Biophys Res Commun. 2003 Jan 3;300(1):141-8.
3.
Processive phosphorylation of p130Cas by Src depends on SH3-polyproline interactions.Pellicena P, Miller WT.J Biol Chem. 2001 Jul 27;276(30):28190-6. Epub 2001 Jun 1.
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