Mouse monoclonal antibody raised against native human AFP.
Immunogen:
Native purified AFP from serum of a hepatoma patient.
Host:
Mouse
Theoretical MW (kDa):
70
Reactivity:
Human
Specificity:
This monoclonal antibody is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin.
Form:
Liquid
Purification:
Protein A/G purification
Isotype:
IgG1, kappa
Recommend Usage:
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)Immunofluorescence (0.5-1 ug/mL)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)The optimal working dilution should be determined by the end user.
Storage Buffer:
In 10 mM PBS.
Storage Instruction:
Store at -20 to -80°C.Aliquot to avoid repeated freezing and thawing.
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq
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