Mouse monoclonal antibody raised against synthetic peptide of human ACTB.
Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to acetylated N-terminus region of human ACTB.
Sequence:
Ac-DDDIAALVV-COOH
Host:
Mouse
Reactivity:
Human, Mouse
Form:
Liquid
Purification:
Protein G purification
Isotype:
IgG1
Recommend Usage:
ELISAFlow CytometryImmunocytochemistryImmunohistochemistry (Frozen sections) (1:100-1:500)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)Western Blot (1:1000-1:5000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.09% sodium azide).
Storage Instruction:
Store at 4°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate with ACTB monoclonal antibody, clone 4C2 (Cat # MAB12986) at 5 ug/mL working concentration.
Immunohistochemistry (Frozen sections)
Immunohistochemical staining (Frozen sections) of 3 days (A) and 1 month (B) old zebrafish embryo with ACTB monoclonal antibody, clone 4C2 (Cat # MAB12986) visualized by IF detection system.
Immunocytochemistry
Immunocytochemistry staining of methanol fixed human dermal fibroblasts with ACTB monoclonal antibody, clone 4C2 (Cat # MAB12986) at 1:500 dilution.
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations:
PS1TP5-binding protein 1,actin, cytoplasmic 1,beta actin,beta cytoskeletal actin
Gene Pathway
Adherens junction
Arrhythmogenic right ventricular cardiomyopathy (ARVC)
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