Rabbit monoclonal antibody raised against acetylated-lysine.
Immunogen:
Acetyllysine conjugated with BSA.
Host:
Rabbit
Specificity:
This antibody reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysine with other modification.
Form:
Liquid
Purification:
Protein A affinity purification
Isotype:
IgG
Recommend Usage:
ELISAFlow CytometryImmunocytochemistry (1:100-1:500)Immunohistochemistry (1:100-1:500)Immunoprecipitation (1:100-1:500)Western Blot (1:500-1:2000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cells nontreated or treated with 400 nM Trichostatin A (TSA) using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat # MAB12823) at 1:2000 dilution.
Immunohistochemistry
Immunocytochemistry
Immunocytochemistry staining of A431 cells (A) and A431 cells treated with Trichostatin A (TSA) (B) using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat # MAB12823) (Red) at 1:500 dilution. Nuclear DNA was stained with DAPI (Blue).
Immunoprecipitation
Immunoprecipitation analysis of Lane 1: A431 cell whole lysates, Lane 2: TSA-treated A431 cell whole lysates; Lane 3: A431 cell whole lysates, Lane 4: TSA-treated A431 cell whole lysates using rabbit IgG antibody; Lane 5: A431 cell whole lysates, Lane 6: TSA-treated A431 cell whole lysates using Acetylated-Lysine monoclonal antibody, clone RM101 (Cat # MAB12823) at 1:500 dilution.
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