Goat monoclonal antibody raised against full length native rabbit IgG Fab.
Immunogen:
Native purified rabbit IgG.
Host:
Goat
Reactivity:
Rabbit
Specificity:
This antibody reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG
Form:
Liquid
Purification:
Protein G purification
Isotype:
IgG
Recommend Usage:
ELISA (0.01 ug/mL- 0.5 ug/mL)Flow CytometryImmunocytochemistry (0.5 ug/mL-2 ug/mL)Immunohistochemistry (0.5 ug/mL-2 ug/mL)ImmunoprecipitationWestern Blot (0.1 ug/mL-0.5 ug/mL)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot
Western blot analysis of Lane 1: nonreduced (-) and Lane 2: reduced (+) rabbit IgG (20 ng/lane) with Rabbit IgG Fab monoclonal antibody, clone RMG01 (Cat # MAB12773) at 0.2 ug/mL working concentration. This antibody reacted to nonreduced rabbit IgG (~150 kDa).
ELISA analysis of IgG from different species with Rabbit IgG Fab monoclonal antibody, clone RMG01 (Cat # MAB12773) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different IgG and the result shows this antibody reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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