Mouse monoclonal antibody raised against human ACTB.
Immunogen:
Recombinant protein corresponding to human ACTB.
Host:
Mouse
Reactivity:
Human, Mouse, Rat
Form:
Liquid
Purification:
Protein G purification
Isotype:
IgG1, kappa
Recommend Usage:
Immunohistochemistry (1:25)Immunofluorescence (1:10-1:50)Western Blot (1:1000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.09% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 whole cell lysates Lane 2: C2C12 whole cell lysates Lane 3: C6 whole cell lysates Lane 4: Hela whole cell lysates Lane 5: MCF-7 whole cell lysates reacted with ACTB monoclonal antibody (Cat # MAB12291) at 1:1000 dilution.
Immunohistochemical staining of formalin-fixed and paraffin-embedded human skeletal muscle section reacted with ACTB monoclonal antibody (Cat # MAB12291) at 1:25 dilution.
Immunofluorescence
Immunofluorescent staining of Hela cell reacted with ACTB monoclonal antibody (Cat # MAB12291) at 1:10-1:50 dilution.
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations:
PS1TP5-binding protein 1,actin, cytoplasmic 1,beta actin,beta cytoskeletal actin
Gene Pathway
Adherens junction
Arrhythmogenic right ventricular cardiomyopathy (ARVC)
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4000-520-616
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