Mouse monoclonal antibody raised against F(ab)2 fragment of Bevacizumab.
Immunogen:
F(ab)2 fragment of Bevacizumab.
Host:
Mouse
Specificity:
This antibodyblocks the Bevacizumab-VEGF interaction in ELISA.
Form:
Liquid
Purification:
Protein G purification
Isotype:
IgG1, kappa
Recommend Usage:
ELISA (2 ug/mL for coating)The optimal working dilution should be determined by the end user.
Storage Buffer:
In TBS, pH 8.0 (0.09% sodium azide)
Storage Instruction:
Store at 4°C for at least 12 months. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
MSDS:
Download
Datasheet:
Download
Publication Reference
1.
Cancer-derived small extracellular vesicles promote angiogenesis by heparin-bound, bevacizumab-insensitive VEGF, independent of vesicle uptake.Ko SY, Lee W, Kenny HA, Dang LH, Ellis LM, Jonasch E, Lengyel E, Naora H.Commun Biol. 2019 Oct 18;2:386. doi: 10.1038/s42003-019-0609-x. eCollection 2019.
2.
Antibody drug quantitation in coexistence with anti-drug antibodies on nSMOL bioanalysis.Iwamoto N, Hamada A, Shimada T.Anal Biochem. 2017 Nov 9;540-541:30-37.
3.
Bioanalysis of bevacizumab and infliximab by high-temperature reversed-phase liquid chromatography with fluorescence detection after immunoaffinity magnetic purification.Todoroki K, Nakano T, Eda Y, Ohyama K, Hayashi H, Tsuji D, Min JZ, Inoue K, Iwamoto N, Kawakami A, Ueki Y, Itoh K, Toyooka T.Anal Chim Acta.2016 Apr 15;916:112-9.
Applications
Enzyme-linked Immunoabsorbent Assay
Blocking
Anti-Bevacizumab monoclonal antibody, clone 2C8 (Cat # MAB11128) inhibits the interaction of Bevacizumab and its antigen VGEF.