产品概述:产品参数 外观:可溶于 DMSO、DMF 的红色固体 Ex/Em:490/523 nm(in MeOH) CAS 号:201860-17-5 储存条件 -20℃避光保存,有效期见外包装。 产品介绍 MitoSceneTM Green I 是一种线粒体绿色荧光染料,可在纳摩尔水平对线粒体进行染色标记,该染料具有质膜透性,且在线粒体膜上积累而呈现明亮的荧光。该染料在线粒体中的定位不依赖于线粒体膜电位,染色固定细胞信噪比不理想,在细胞固定、透化之后,会导致荧光信号减弱或丢失,所以我们推荐只用来染活细胞。注意事项 1. 荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。 2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
说明书:UE-M4063S/M4063L MSDS:MSDS M4063 MitoSceneTM Green I
使用本产品的文献:1.Circadian clock gene NPAS2 promotes reprogramming of glucose metabolism in hepatocellular carcinoma cellsPengYuana,TaoYang,JiaoMu,JingZhao,YiYang,ZhaoyongYan,YiranHou,ChengChen,JinliangXing,HongxinZhang,JibinLiCancer Letters Volume 469, 28 January 2020, Pages 498-509参考文献1.Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green, and MitoTracker Green is affected by mitochondrial membrane potential altering drugs.应用方向:分析HL60细胞线粒体膜电位2.Efficacy of MitoTracker Green and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissUEs.应用方向:对线粒体特异染色,线粒体形态和功能的研究3.MitoTracker Green labeling of mitochondrial proteins and their subseqUEnt analysis by capillary electrophoresis with laser-induced fluorescence detection.应用方向:标记线粒体是通过与线粒体的蛋白特异结合4.Mitotracker green is a P-glycoprotein substrate.应用方向:染料是一种P-糖蛋白底物5.Alveolar macrophages from subjects with chronic obstructive pulmonary disease are deficient in their ability to phagocytose apoptotic airway epithelial cells.应用方向:追踪16HBE气道上皮细胞的有丝分裂