Description:
HistoneH3combineswithHistoneH4toformtheH3/H4tetramer.TwoH2A/H2BheterodimersinteractwithanH3/H4tetramertoformthehistoneoctamer.Itisalsomodifiedbyvariousenzymesandcanactasasubstrateforthem.Thesemodificationshavebeenshowntobeimportantingeneregulation.
HistoneH3.1,anH3variantthathasthusfaronlybeenfoundinmammals,isreplicationdependentandisassociatedwithgeneactivationandgenesilencing.
ProteinSequence: ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA(Genbankaccessionnumber:AAN10051)
ProductSource
AnE.colistrainthatcarriesaplasmidencodingtheclonedhumanhistoneH3.1gene,HIST1H3AorH3FA.(Genbankaccessionnumber:AF531274)Notes:
WhenrunningSDS-PAGEgels,HistoneH3.1canself-oligomerizeiffreshlypreparedDTT(dithiothreitol)isnotusedwhenpreparingthesampleforgelelectrophoresis.1mg/ml,65µMiscalculatedusingthemolarextinctioncoefficientforHistoneH3.1(4080)anditsabsorbanceat280nm(3,4).1.0A280units=3.8mg/ml