The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature . (25°C)
For details on NEB"s quality controls for DNA ligases, visit our Ligase Quality page.Ligation Time Course:LITMUS 28i vector was cut with either EcoRV (blunt) or HindIII (cohesive), treated with calf intestinal phosphatase and gel purified. Blunt inserts from a HaeIII digest of ΦX174 DNA and cohesive inserts from a HindIII digest of λ DNA were ligated into the respective vectors at a 3:1 insert:vector ratio using the Quick Ligation Kit. Ligation products were transformed into chemically competent E. coli DH-5α cells and grown overnight on LB-amp plates at 37°C.
1X Quick Ligation Reaction Buffer:66 mM Tris-HCl10 mM MgCl21 mM Dithiothreitol1 mM ATP7.5% Polyethylene glycol (PEG6000)pH 7.6 @ 25°C
.
This product is related to the following categories:
DNA Ligases Products
This product can be used in the following applications:
Cloning Ligation,
Fast Cloning: Accelerate your cloning workflows with reagents from NEB