EnGen® Sau Cas9 from Staphylococcus aureus is an RNA-guided DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA. Targeting requires a ~100 nucleotide guide RNA (gRNA) complementary to the target site, as well as a 5′ NNGRRT 3′ (N = any, R = A or G) protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. DNA cleavage by EnGen Sau Cas9 occurs ~3 bases 5′ of the PAM. EnGen Sau Cas9 has Simian virus 40 (SV40) T antigen nuclear localization signals (NLS) at both the N- and C-termini of the protein.
Product Source
EnGen® Sau Cas9 from Staphylococcus aureus is expressed as a C-terminal 6X His-tagged fusion in E. coli.
This product is related to the following categories:
Genome Editing Products,
Programmable Nucleases
This product can be used in the following applications: