NudC is a NUDIX pyrophosphatase that efficiently hydrolyzes NAD+- and NADH-capped RNA, generating a ligatible 5′ monophosphate on the RNA (NAD+ decapping or deNADding) (1). Deletion of the nudC gene has been shown to increase the fraction of NAD+-capped RNA in E. coli (2).NudC has also been shown to hydrolyze small molecules containing an ADP moiety such as NADH, NAD+, AppA, ADP-ribose, ADP-glucose and metabolic co-factors such as FAD, Coenzyme A and Acetyl CoA at varying efficiency (3 and unpublished results). With its activity on various dinucleotides and metabolic co-factors, NudC may potentially be used to identify RNA carrying non-canonical caps by pyrophosphorolysis of the cap and analysis of the product nucleotide, or identifying the resulting 5" monophosphate end of the RNA using 5’ ligation-based methods such as RNA-seq or 5’ RACE (4).
This product is related to the following categories:
RNA Modification Products,
RNA Reagents Products
This product can be used in the following applications:
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