*Methylation Conditions: Methylation of 1 µg substrate with 5 U EcoGII Methyltransferase in 1X rCutSmart™ Buffer supplemented with 160 µM SAM for 60 minutes at 37°C. Following methylation, samples were column purified, digested to nucleosides and analyzed by LCMS to determine the ratio of N6mA to A (% methylation).1 Tested substrates include: plasmid DNA (pBR322 #N3033, pUC19 #N3041), genomic DNA (Human Embryonic Kidney (HEK293) cells, Mouse Ear and Tail, Rat Liver and Kidney, λ DNA (dam-) #N3013), ds DNA oligo (80 nucleotides), ss DNA oligo (60 nucleotides), DNA/RNA hybrid (80 nucleotides), total RNA (HeLa cell RNA, Mouse Kidney RNA, Rat Brain RNA), mRNA (in vitro transcribed 1.8 kb FLuc mRNA). 2 Methylation levels of >20% have been observed by using less substrate (250 ng) and increasing the amount of enzyme (15 U). Methylation levels can be increased by decreasing the amount of substrate, increasing the amount of enzyme and/or increasing the length of the methylation reaction.
Product Source
An E. coli strain that carries the cloned EcoGII methyltransferase gene from E. coli (strain C227-11)(1).
This product is related to the following categories:
购物车为空，快去购物吧！
4000-520-616
咨询顾问
特别 提示
