NEBNext Q5U Master Mix is optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification.The polymerase component of the master mix, Q5U, is a modified version of Q5 High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. The NEBNext Q5U Master Mix is an aptamer-based hot start formulationthat allows simple room temperature reaction set up. The convenient 2X master mix format contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification.NEBNext Q5U Master Mix is included as a component in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) and is also compatible with library amplification in bisulfite sequencing workflows.
This product is related to the following categories:
Epigenetic Analysis,
Q5® High-Fidelity DNA Polymerases Products,
Epigenetics Products,
PCR, qPCR & Amplification Technologies Products
This product can be used in the following applications:
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