当前位置 : NEB >>> NEB/NEBNext® Q5® Hot Start HiFi PCR Master Mix/50 reactions/M0543S
NEB/NEBNext® Q5® Hot Start HiFi PCR Master Mix/50 reactions/M0543S
  • NEB/NEBNext® Q5® Hot Start HiFi PCR Master Mix/50 reactions/M0543S

NEB/NEBNext® Q5® Hot Start HiFi PCR Master Mix/50 reactions/M0543S

价格: 试用 市场价: 0.00

货号: M0543S
品牌: NEB
规格
数量
库存(0)
特别 提示
代购产品:无质量问题不接受退换货,下单前请仔细核对信息。
下单后请及时联系客服核对商品价格,订单生效后再付款。
资深产品顾问
咨询顾问

全国免费服务热线

4000-520-616


  • 自营商城 一站式服务
  • 厂家直采 剔除溢价
  • 品质甄选 正品保证
  • 严控流程 只做188精品
  • 极速物流 如约送货
  • 详情
  • 使用说明
  • 常见问题
    • NEBNext® Q5 Hot Start HiFi PCR Master Mix is the original hot start NEBNext formulation of Q5 DNA polymerase. This formulation is also included as a component in all of the original NEBNext Ultra kits, for DNA and RNA library preparation.The NEBNext Ultra II Q5 Master Mix (NEB #M0544) is now available, which further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.The polymerase component of both master mixes, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has the highest fidelity available (> 280-fold higher than that of Taq DNA Polymerase), resulting in ultra-low error rates.This convenient 2X master mix contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification. The inclusion of the hot start aptamer allows convenient room temperature reaction set up.

      Depth of coverage correlation with unamplified librariesDepth of coverage correlation with unamplified libraries
      Average depth of coverage of each 10 kb window of the hg19 reference genome is plotted for NEBNext Q5 Hot Start HiFi PCR Master Mix on the Y axis, and correlated with the number of reads from a library prepared without an amplification step (PCR-Free) on the X axis. Libraries were prepared using the NEBNext Ultra DNA Library Prep Kit (NEB #E7370), sequenced on a HiSeq® 2500 mapped to the hg19 reference genome (Bowtie 2.1.0), duplicate marked (Picard 1.56), and downsampled to 420M reads (Picard 1.56). Reads on each 10 kb interval of hg19 were counted using Bedtools (2.19.1), and average coverage was calculated by dividing read bases the interval length (10 kb).
      NEBNext Q5 Hot Start HiFi PCR Master Mix minimizes GC biasNEBNext Q5 Hot Start HiFi PCR Master Mix minimizes GC bias
      Libraries of human (IMR-90) or E. coli K12 genomic DNA were unamplified (“PCR-free”) or amplified using NEBNext Q5 Hot Start HiFi PCR Master Mix, and sequenced on an Illumina MiSeq®. GC bias plots were generated, with %GC content of 100 bp windows on the X axis. Normalized coverage is indicated by the blue circles, %GC of the reference sequence indicated by the red lines and base quality at %GC indicated by the green line. Similar GC bias was observed for the libraries amplified with NEBNext Hot Start HiFi PCR Master Mix compared to PCR-free libraries.
      Reaction ConditionsNEBNext Q5 Hot Start HiFiPCR Master Mix, DNA template and 0.5 μM to1.25 μM primers (depending on sample input) in atotal reaction volume of 50 μl.

      Product Source

      An E. coli strain that carries the Q5 DNA Polymerase gene.
      This product is related to the following categories:
      Q5® High-Fidelity DNA Polymerases Products,
      Master Mixes Products,
      Next Generation Sequencing Library Preparation
      This product can be used in the following applications:
      Polymerases for NGS Library Preparation,
      Hot Start PCR,
      Fast PCR,
      Specialty PCR,
      Routine PCR,
      DNA Amplification, PCR & qPCR,
      PCR
    售后保障
    蚂蚁淘生物188,秉承蚂蚁淘一贯的严谨态度,由蚂蚁淘公司专业人员负责品控、采购、物流、销售、售后,保障正品优质。以“快速好省,为科研提供好产品、好价格”为理念,直接链接原厂家,从全国各地原制造商严格挑选188款科研精品,剔除品牌溢价,188生物新电商,把好的产品带给科研!  力求给你最优质的商品。
  • Q:生物188产品正品保障吗?
    A:生物188质量把控人员具有十年的从业经验,在业界享有良好的口碑;自营商城,直接从厂家采购, 自己的团队负责国际物流和清关,中间没有第三方,所有流程严格把控,100%保证正品,假一罚十。

    Q:下单后可以修改订单吗?
    A:下单后的商品付款之前可以修改;订单付款成功,需要联系我们客服进行修改;客服电话:4000-520-616

    Q:可以开发票吗?
    A:本网站所售商品都是正规清关,均开具16%正规发票,发票金额含配送费金额,另有说明的除外。

    Q:商品几天可以发货?
    A:生物188商品,全部现货销售,付款后即可发货,一般一周内送达!

    Q:如何联系商家?
    A:有任何问题够可以电话咨询我们,全国24小时免费服务热线:4000-520-616 或联系我们的在线客服QQ:1570468124

    Q:收到的商品少了/发错了怎么办?
    A:同个订单购买多个商品可能会分为一个以上包裹发出,可能不会同时送达,建议查看订单详情是否是 部分发货状态;如未收到,可联系在线客服或者致电4000-520-616。

    Q:退换货/维修需要多长时间?
    A:一般情况下,退货处理周期为客户收到产品一个月内(以快递公司显示签收时间为准),包装规格、 数量、品种不符,外观毁损、短缺或缺陷,请在收到货24小时内申请退换货;特殊商品以合同条款为准。

何为188

极简而严谨,我们仅销售188款生物医学科研用品,款款都是爆款;因为少所以聚焦,聚焦甄选每一款产品,聚焦服务每一位客户!

关注我们 :

点击QQ联系我们
生物188微信

关注188微信公众号

获取最新优惠活动通知