Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5′ and 3′ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- and deoxyribonucleoside triphosphates (NTPs and dNTPs). Quick CIP is useful in many applications that require the dephosphorylation of DNA or RNA ends. In cloning, dephosphorylation prevents re-ligation of linearized plasmid DNA. The enzyme can quickly dephosphorylate 5′ protruding, 5′ recessed, and blunt ends in just 10 minutes. Quick CIP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis.
Quick CIP is completely and irreversibly inactivated by heating it at 80°C for 2 minutes, unlike wild type CIP, which is not heat-inactivatable. This makes removal of Quick CIP prior to ligation or end-labeling unnecessary.
Product Source
A Pichia pastoris clone that carries the calf intestinal alkaline phosphatase gene from calf intestinal mucosa. - This product is related to the following categories:
- Phosphatases Products,
- RNA Modification Products
- This product can be used in the following applications:
- Dephosphorylation,
- RNA Modification,
- In vitro Transcription for RNA Synthesis,
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