Description:
OneTaq®HotStartQuick-Load®2XMasterMixwithGCBufferisanoptimized,ready-to-useblendofTaqandDeepVentR™DNAPolymerasescombinedwithanaptamer-basedinhibitor.ThisenzymeblendisideallysuitedtoPCRapplicationsfromGC-richtemplates,includingpureDNAsolutions,bacterialcolonies,andCDNAproducts.The3´→5´exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).Thehotstartnatureoftheenzymeoffersconveniencewithdecreasedinterferencefromprimerdimersandsecondaryproducts.TheconvenientmastermixformulationcontainsdNTPs,MgSO4,buffercomponentsandstABIlizersaswellastwocommonlyusedtrackingdyesforDNAgels.Ona1%agarosegelin1XTBE,XyleneCyanolFFmigratesat~4kbandTartrazinemigratesat~10bp.Bothdyesarepresentinconcentrationsthatdonotmaskco-migratingDNAbands.
Highlights
ProductSource
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andanE.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
OneTaq HighGCEnhancer | | 10X |
Notes:
ProductspecificationsforindividualcomponentsintheOneTaqHotStartQuick-Load2XMasterMixwithGCBufferareavailableseparately.OneTaqHotStartQuick-Load2XMasterMixwithGCBufferisstableforfifteenfreeze-thawcycleswhenstoredat-20°C.OneTaqHotStartQuick-Load2XMasterMixwithGCBufferisalsostableforonemonthat4°C,soforfrequentuse,analiquotmaybekeptat4°C.