Description:
OneTaq®HotStartQuick-Load®2XMasterMixwithStandardBufferisanoptimized,ready-to-useblendofTaqandDeepVentR™DNAPolymerasescombinedwithanaptamer-basedinhibitor.ThisenzymeblendisideallysuitedtoroutinePCRapplicationsfromavarietyoftemplates,includingpureDNAsolutions,bacterialcoloniesandCDNAproducts.The3´→5´exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).Thehotstartnatureoftheenzymeoffersconveniencewithdecreasedinterferencefromprimerdimersandsecondaryproducts.TheconvenientQuick-LoadmastermixformulationcontainsdNTPs,MgCl2,buffercomponentsandstABIlizersaswellastwocommonlyusedtrackingdyesforDNAgels.Ona1%agarosegelin1XTBE,XyleneCyanolFFmigratesat~4kbandTartrazinemigratesat~10bp.Bothdyesarepresentinconcentrationsthatdonotmaskco-migratingDNAbands.
ProductSource
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andanE.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.Notes:
ProductspecificationsforindividualcomponentsintheOneTaqHotStartQuick-Load2XMasterMixwithStandardBufferareavailableseparately.OneTaqHotStartQuick-Load2XMasterMixwithStandardBufferisstableforfifteenfreeze-thawcycleswhenstoredat-20°COneTaqHotStartQuick-Load2XMasterMixwithStandardBufferisalsostableforonemonthat4°C,soforfrequentuse,analiquotmaybekeptat4°C.