Description:
OneTaq®Quick-Load®2XMasterMixwithStandardBufferisanoptimized,ready-to-useblendofTaqandDeepVentR™DNAPolymerasesideallysuitedtoroutinePCRapplicationsfromavarietyoftemplates,includingpureDNAsolutions,bacterialcoloniesandCDNAproducts.The3´→5´exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).Theconvenientquick-loadmastermixformulationcontainsdNTPs,MgCl2,buffercomponentsandstABIlizersaswellastwocommonlyusedtrackingdyesforDNAgels.Ona1%agarosegelin1XTBE,XyleneCyanolFFmigratesat~4kbandTartrazinemigratesat~10bp.BothdyesarepresentinconcentrationsthatdonotmaskcomigratingDNAbands.
ProductSource
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andanE.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.Notes:
ProductspecificationsforindividualcomponentsintheOneTaqQuick-Load2XMasterMixwithStandardBufferareavailableseparately.OneTaqQuick-Load2XMasterMixwithStandardBufferisstableforfifteenfreeze-thawcycleswhenstoredat-20°C.OneTaqQuick-Load2XMasterMixwithStandardBufferisalsostableforonemonthat4°C,soforfrequentuse,analiquotmaybekeptat4°C.