Description:
OneTaq®HotStart2XMasterMixwithGCBufferisanoptimizedblendofTaqandDeepVent™DNAPolymerasescombinedwithanaptamer-basedinhibitor.ThisenzymeblendisideallysuitedtoPCRapplicationsfromGC-richtemplates,includingpureDNAsolutions,bacterialcoloniesandCDNAproducts.The3´→5´exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).Thehotstartnatureoftheenzymeoffersconveniencewithdecreasedinterferencefromprimerdimersandsecondaryproducts.TheconvenientmastermixformulationcontainsdNTPs,MgSO4, buffercomponentsandstABIlizers,requiringonlytheadditionofprimersandDNAtemplateforrobustamplification.
ProductSource
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andan E.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
OneTaq HighGCEnhancer | | 50% |
Notes:
OneTaqHotStart2XMasterMixwithGCBufferisstableforfifteenfreeze-thawcycleswhenstoredat-20°C.OneTaqHotStart2XMasterMixwithGCBufferisalsostableforonemonthat4°C,soforfrequentuse,analiquotmaybekeptat4°C.ProductspecificationsforindividualcomponentsintheOneTaqHotStart2XMasterMixwithGCBufferareavailableseparately.