Description:
OneTaq®HotStart2XMasterMixwithStandardBufferisanoptimizedblendofTaqandDeepVentR™DNAPolymerasescombinedwithanaptamer-basedinhibitor.ThisenzymeblendisideallysuitedtoroutinePCRapplicationsfromavarietyoftemplates,includingpureDNAsolutions,bacterialcoloniesandCDNAproducts.The3´→5´exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).Thehotstartnatureoftheenzymeoffersconveniencewithdecreasedinterferencefromprimerdimersandsecondaryproducts.TheconvenientmastermixformulationcontainsdNTPs,MgCl2andotherbuffercomponentsandstABIlizerslistedbelow,requiringonlytheadditionofprimersandDNAtemplateforrobustamplification
ProductSource
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andan E.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.Notes:
ProductspecificationsforindividualcomponentsintheOneTaqHotStart2XMasterMixwithStandardBufferareavailableseparately.OneTaqHotStart2XMasterMixwithStandardBufferisstableforfifteenfreeze-thawcycleswhenstoredat-20°C.OneTaqHotStart2XMasterMixwithStandardBufferisalsostableforonemonthat4°C,soforfrequentuse,analiquotmaybekeptat4°C.