Description:
OneTaq®2XMasterMixwithStandardBufferisanoptimizedblendofTaqandDeepVentR™DNAPolymerasesideallysuitedtoroutinePCRapplicationsfromavarietyoftemplatesincludingpureDNAsolutions,bacterialcolonies,andCDNAproducts.The3´→5´exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).TheconvenientmastermixformulationcontainsdNTPs,MgCl2,buffercomponentsandstABIlizers,requiringonlytheadditionofprimersandDNAtemplateforrobustamplification.
ProductSource
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andanE.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.Notes:
OneTaq2XMasterMixwithStandardBufferisstableforfifteenfreeze-thawcycleswhenstoredat-20°C.OneTaq2XMasterMixwithStandardBufferisalsostableforonemonthat4°C,soforfrequentuse,analiquotmaybekeptat4°C.ProductspecificationsforindividualcomponentsintheOneTaq2XMasterMixwithStandardBufferareavailableseparately.