Description:
OneTaqDNAPolymeraseisanoptimizedblendofTaqandDeepVentR™DNApolymerasesforusewithroutineanddifficultPCRexperiments.The3´→5´exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).TheOneTaqReactionBuffersandHighGCEnhancerhavebeenformulatedforrobustyieldswithminimaloptimization,regardlessofatemplate’sGCcontent.
OneTaqDNAPolymeraseissuppliedwithtwo5Xbuffers:(StandardandGC),aswellasaHighGCEnhancersolution.Formostroutineand/orAT-richamplicons(LamBDa,etc.)orcomplexampliconswithupto~65%GCcontent,OneTaqStandardReactionBufferprovidesrobustamplification.ForGC-richamplicons,theOneTaqGCReactionBuffercanimprovebothperformanceandyield.ForparticularlyhighGCordifficultamplicons,theOneTaqHighGCEnhancercanbeaddedatafinalconcentrationof10–20%toreactionscontainingOneTaqGCReactionBuffer.
ProductSource
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andanE.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
OneTaq® GCReactionBuffer | -20 | 5X |
OneTaq®StandardReactionBuffer | -20 | 5X |
OneTaq HighGCEnhancer | | 5X |
Notes:
TheOneTaqHighGCEnhancershouldnotbeusedalone.ItshouldbeaddedonlytoreactionswiththeOneTaqGCReactionBufferandwilltypicallyimproveyieldswhenotherconditionshavefailed.ProductspecificationsforindividualcomponentsintheOneTaqDNAPolymerasemixareavailableseparately.