Cas9 Nuclease, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) (1). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.
Product Source
A His-tagged engineered variant of E. coli that carries the cloned Cas9 gene from Streptococcus pyogenes.
This product is related to the following categories:
Genome Editing Products,
Programmable Nucleases
This product can be used in the following applications: