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特别 提示
Bst 3.0 DNA Polymerase is anin silico designed homologue of Bacillusstearothermophilus DNA Polymerase I, LargeFragment engineered and fused to a novel nucleic acid binding domain for improved isothermalamplification performance and increased reversetranscription activity. Bst 3.0 DNA Polymerasecontains 5´→3´ DNA polymerase activity witheither DNA or RNA templates and strong stranddisplacement activity, but lacks 5´→3´ and 3´→5´exonuclease activity. Bst 3.0 DNA Polymerasedemonstrates robust performance even in highconcentrations of amplification inhibitors, including dUTP andfeatures significantly increased reverse transcriptaseactivity compared to Bst DNA Polymerase.
An example of the utility and speed offered by Bst 3.0 DNA Polymerase can be seen in this publication that explores the development of a novel digital LAMP assay: Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples.
