T4 RNA Ligase 2, truncated KQ (T4Rnl2tr R55K, K227Q) specifically ligates the preadenylated5´ end of DNA or RNA to the 3´ OH endof RNA. The enzyme does not use ATP for ligationbut requires pre-adenylated linkers.T4 Rnl2tr R55K, K227Q is a double-point mutant ofT4 RNA Ligase 2, truncated (NEB #M0242). Mutationof K227 in T4 RNA Ligase 2 reduces enzymelysyl adenylation (1). K227Q reduces the formationof undesired ligation products (concatemers andcircles) by T4 Rnl2tr (2,3), by reducing the traceactivity of T4 Rnl2tr in transfer of adenylyl groupsfrom linkers to the 5´-phosphates of input RNAs(3). Mutation of R55K in T4 Rnl2tr K227Q increasesthe ligation activity of the enzyme to levels similarto T4 Rnl2tr (3).The exclusion of ATP, use of pre-adenylated linkers,and the reduced enzyme lysyl adenylation activityprovide the lowest possible background in ligationreactions. This enzyme has been used for optimizedlinker ligation for high-throughput sequencinglibrary construction of small RNA (4).
Product Source
T4 Rnl2tr R55K, K227Q is expressed as an MBP fusion from a plasmid in E. coli which encodes the first 249 amino acids of the full length T4 RNA Ligase 2 with an arginine to lysine and a lysine to glutamine mutations at positions 55 and 227, respectively.
This product is related to the following categories:
RNA Ligases Products,
RT-PCR Products,
cDNA Synthesis & Reverse Transcriptases Products
This product can be used in the following applications:
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