Hemo KlenTaq is a truncated version of Taq DNA Polymerase, lacking the first 280 amino acids (1). Hemo KlenTaq also contains mutations that make it resistant to inhibitors present in whole blood. It can amplify directly from whole blood from humans and mice. Hemo KlenTaq tolerates up to 20% whole blood in a 25 µl reaction (30% in 50 µl reaction).
Amplification of human whole blood with Hemo KlenTaq. Percent blood present in sample and anticoagulant used are indicated in the legend. Ladder M is the 2-Log DNA Ladder (NEB #N3200).
Product Source
AnE. colistrain that carries a mutantTaqDNA polymerase gene. The protein lacks the N-terminal 5´→ 3´ exonuclease domain and the gene has three internal point mutations.
This product is related to the following categories:
Other PCR Polymerases
This product can be used in the following applications: