T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch. The protein is the product of T7 gene 3.
Highlights
Isolated from a recombinant source
Supplied with 10X Reaction Buffer
Recognizes non-perfectly matched DNA
Product Source
An E. coli strain that carries a fusion of maltose binding protein and T7 Endonuclease I (T7 Endo I).
This product is related to the following categories:
Mutation Detection Reagents and Kits,
Genome Editing Products,
Exonucleases and Non-specific Endonucleases Products,
DNA Repair Enzymes and Structure-specific Endonucleases Products
This product can be used in the following applications: