Description:
TmaEndonucleaseIIIisaThermostablehomologofE.coliEndonucleaseIII,theproductoftheE.colinthgene(1).ItpossessesbothN-glycosylaseandAP-lyaseactivities.TheN-glycosylaseactivityreleasesdamagedpyrimidinesfromdouble-strandedDNA,generatinganabasicsite.TheAP-lysaseactivitycleavesthephosphodiesterbond3´totheabasicsiteresultingina3´-α,β-unsaturatedaldehydeanda5´-phosphate.
Damagedbases recognized andremovedby Tma EndonucleaseIIIinclude 5,6 dihydroxythymine,thymineglycoland abasicsites (2).
ProductSource
ClonedfromThermotogamaritimaandoverexpressedinE.coli.ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
ThermoPol® ReactionBufferPack | -20 | 10X |
Notes:
DiluentCompatibility: DiluentD100mMKCl,10mMTris-HCl(pH7.4@25°C),0.1mMEDTA,1mMDTT,0.1%TritonX-100and50%glycerol.