Endonuclease III (Nth) protein from E.coli acts as both N-glycosylase and a AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating a basic (AP site). The AP-lyase activity of the enzyme cleaves 3´ to the AP site leaving a 5´phosphate and a 3´ring opened sugar. Some of the damaged bases recognized and removed by Endouclease III include urea, 5, 6 dihydroxythymine, thymine glycol, 5-hydroxy-5 methylhydanton, uracil glycol, 6-hydroxy-5, 6-dihdrothimine and methyltartronylurea (1,2).
Product Source
An E. coli strain which carries the cloned nth gene from Escherichia coli.
This product is related to the following categories:
DNA Repair Enzymes and Structure-specific Endonucleases Products
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