Vent® DNA Polymerase is a high-fidelity thermophilic DNA polymerase. The fidelity of Vent DNA Polymerase is 5-15-fold higher than that observed for Taq DNA Polymerase (1,2). This high fidelity derives in part from an integral 3"→5" proofreading exonuclease activity in Vent DNA Polymerase (1,3). Greater than 90% of the polymerase activity remains following a 1 hour incubation at 95°C.Amplification of Jurkat genomic DNA with Vent DNA Polymerase. Amplicon sizes are indicated below gel. Marker M is the 1 kb DNA Ladder (NEB #N3232 ).
Product Source
AnE. colistrain that carries the Vent® DNA Polymerase gene fromThermococcus litoralis(4). The native organism is capable of growth at up to 98°C and was isolated from a submarine thermal vent (5).
This product is related to the following categories:
Other PCR Polymerases
This product can be used in the following applications: