Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37° C – 75° C).
Product Source
Purified from anE. colistrain containing the cloned ligase gene fromThermus thermophilus HB8 (1-3).
This product is related to the following categories:
DNA Ligases Products
This product can be used in the following applications: