E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated. E. coli DNA Ligase is active at a range of temperatures (4° C – 37° C).
Highlights
Specific Activity: 6,000 units/mg
Supplied with 10X Reaction Buffer
Product Source
Purified fromE. colistrain containing a cloned E. coli DNA Ligase gene.
This product is related to the following categories:
DNA Ligases Products
This product can be used in the following applications: