Description
Lipofectamine®3000TransfectionReagentleveragesourmostadvancedlipidnanoparticletechnologytoprovidesuperiortransfectionperformancewithimprovedapplicationoutcomesandreproduc
IBLeresults.Thisreagentdeliverssuperiortransfectionefficiencyandimprovedcellvi
ABIlityforthewidestrangeofhard-to-transfectandcommoncells(e.g.,HEK293,HeLa).
Successfullytransfectthewidestvarietyof
BIOLOGicallyrelevantcelltypeswithareagentthatoffers:
•Superiorperformance—ourhighestefficiencyreagentfordifficult-to-transfectcells
•Improvedcellviability—gentleonyourcells,withlowtoxicity
•Versatile—onereagentforDNA,RNA,andco-transfection
EfficientlyTransfectDifficult-to-TransfectCellsLipofectamine®3000reagentisdesignedtoefficientlytransfectdifficult-to-transfectcells,yieldingsuperiortransfectionperformanceacrossthebroadestarrayofcelltypes.Successfullytransfectedcelltypesinclude:
Fibroblast(3T3,COS-7) | Myoblast(C2C12,L6CRL-1458) |
Hepatocyte(HepG2,HuH7) | Erythroleukemiccell(K562) |
Breastcarcinoma(MCF7,Hs578T) | Prostatecarcinoma(LNCap) |
Lungcarcinoma(A549,NCI-H460) | Osteosarcoma(U-2OS,Saos-2) |
Coloncarcinoma(Caco2,SW480) | Pancreaticcarcinoma(PANC-1) |
Skinmelanoma(SK-MEL-28) | |
Viewperformancedatafor60+celllinespluscomparisontoLipofectamine2000>
TransfectCommonCellsEfficientlyandCost-EffectivelyForcommoncelltypes,Lipofectamine®3000reagentismorecost-effectivethanotherreagentsduetoitshighconcentrationandlowusageamount.The1.5mLsizeprovidesupto1500transfectionreactions(in24-wellplates).
AchieveSuperiorTransfectionEfficiencyWhileMaintainingLowToxicityLipofectamine®3000reagentmaintainsahightransfectionefficiencywithinarobustdynamicrangeoflipiddosesforquickandeasyoptimization.Alowtoxicitylipiddose(0.75µLfor24-wellplates)issuggestedforapplicationsrequiringminimaldisruptionofthecells.
EnableHighTransfectionEfficiencyinNovelGenomeEditingApplicationsLipofectamine®3000reagentissuitablefornovelgenomeeditingapplications.ItincreasesthelikelihoodofsuccessfulcleavageandrecombinationwithTALENsorCRISPRsthroughhighlyefficienttransfection,ultimatelymaximizingtheefficiencyofgeneticmodificationsandsimplifyingthedownstreamprocesses.
ForResearchUseOnly.Notforuseindiagnosticprocedures.