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Pierce/Amyloid beta 40 Mouse ELISA Kit/96 tests/KMB3481
  • Pierce/Amyloid beta 40 Mouse ELISA Kit/96 tests/KMB3481

Pierce/Amyloid beta 40 Mouse ELISA Kit/96 tests/KMB3481

价格: ¥7200.00 市场价: 12000.00

货号: KMB3481
品牌: Pierce
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    • Description

      TheMouseBetaAmyloid1-40(Aβ40)ELISAresearchuseonlykitistobeusedforthequantitativedeterminationofMouseandRatAβ40insamples(e.g.,cellculturesupernatants,tissuehomogenates,cerebrospinalfluid[CSF],etc.)using96-wellplatesandamicroplatereader.TheassaywillrecognizebothnaturalandsyntheticformsofMouseandRatAβ40.Theanti-mouseAβ40antibodyusedinthiskitiscapableofselectivelydetectingAβ40andnotAβ42orAβ43.

      Performancecharacteristics
      •Sensitivity:<5>
      •Standardcurverange:7.81–500pg/mL
      •Sampletypes:cellculturesupernatants,tissuehomogenates,cerebrospinalfluid(CSF)
      •Speciescross-reactivity:mouse,rat
      •Samplevolume:100μL(prediluted)
      •Totalassayincubationtime:4hrs

      Principleofthemethod
      TheMouseAβ40kitisasolidphasesandwichEnzyme-LinkedImmunosorbentAssay(ELISA).AmonoclonalantibodytotheNH2-terminusofMouseAβhasbeencoatedontothewellsofthemicrotiterstripsprovided.Samples,includingstandardsofknownMouseAβ40content,controlspecimensandunknowns,arePipettedintothesewells.TheAβantigenbindstotheimmobilized(capture)antibody.Afterwashing,arabbitmonoclonalantibodyspecificfortheCOOH-terminusofthe1-40Aβsequenceisaddedtothewells.BoundrabbitantibodyisdetectedbytheuseofahorserADIshperoxidase-labeledanti-rabbitantibody.

      Afterremovalofexcessanti-rabbitantibody,asubstratesolutionisadded,whichisacteduponbytheboundenzymetoproducecolor.TheintensityofthiscoloredproductisdirectlyproportionaltotheconcentrationofMouseAβ40presentintheoriginalspecimen.

      Backgroundinformation
      Alzheimer’sDisease(AD)ischaracterizedbythepresenceofextracellularplaquesandintracellularneurofibrillarytangles(NFTs)inthebrain.Themajorproteincomponentoftheseplaquesisbetaamyloidpeptide(Aβ),a40to43aminoacidpeptidecleavedfromamyloidprecursorproteinbyβ-secretase(e.g.,BACE)andaputativeγ(gamma)secretase.Increasedreleaseofthe‘longerforms’ofAβpeptide,Aβ42orAβ43,whichhaveagreatertendencytoaggregatethanAβ40,occursinindividualsexpressingcertaingeneticmutations,expressingcertainApoEalleles,ormayinvolveother,stillundiscovered,factors.ManyresearcherstheorizethatitisthisincreasedreleaseofAβ42/Aβ43whichleadstotheabnormaldepositionofAβandtheassociatedneurotoxicityinthebrainsofaffectedindividuals.

      EachMouseAβ40ELISAkitisvalidatedforsensitivity,specificity,precision,andlot-to-lotconsistency.Seeproductinsertformoreinformationonvalidation.

      Relatedlinks
      LearnmoreaboutELISAkits
      Learnmoreaboutotherimmunoassays

      *PleaserefertotheELISAprotocolforspecificreferencecitations.
      ForResearchUseOnly.Notforuseindiagnosticprocedures.

      Figures

      Specifications

      AssayIncubationTime:4hr
      AssayRange:7.81-500pg/mL
      AssaySensitivity:<5pg/mL
      DetectionMethod:Colorimetric
      ForUseWith(Equipment):MicroplateReader
      FormerIdentifiers:Novex
      GeneAliases:Abeta,Abpp,Adap,Ag,Cvap,E030013M08Rik,betaApp
      GeneID:11820
      GeneName:amyloidbeta(A4)precursorprotein
      GeneSymbol:APP
      ImmunoassayKitFormat:CoatedELISAKit
      LabelorDye:HRP(HorseradishPeroxidase)
      ProductSize:96tests
      ProteinName:AmyloidbetaA4protein
      SampleType(Specific):CellCultureSupernatants, CerebrospinalFluid, TissueHomogenates
      SampleVolume:100µL
      ShippingCondition:WetIce
      Target(KitNamed):Amyloidbeta40
      TargetSpecies(Named):Mouse
      TargetSpecies(Validated):Mouse, Rat
      UniProtID:P12023

      Contents&storage

      Pre-coatedplate(s),standardorcalibrator,detectorantibody,HRPconjugate,diluents,washbuffer,substrate(s),andstopsolution.Storekitat2-8°C.SeeproductmanualfordetailedcontentsandstorageconditionsformaximumstABIlity.
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