2-NBDG Glucose Uptake Assay Kit provides a sensitive and non-radioactive assay for measuring glucose uptake in cultured cells. The fluorescence signal can be monitored by fluorescence microscope or flow cytometer with a 488 nm laser and 530/30 nm emission filter (FITC channel). This Assay Kit is the most robust tool for monitoring glucose transporters.
Storage Instruction:
Store the kit at -20°C and avoid from light.
Note:
Example Data Analysis and Figures.
Fluorescence images of 2-NBDG uptake in CHO-K1 cells using the 2-NBDG Glucose Uptake Assay Kit. CHO-K1 cells at 40,000 cells/well/100 uL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with 20 mM Glucose (B) or 100 uM Phloretin (C) at 37°C for 1 hour, then incubated with 100 uM 2-NBDG staining solution for 20 minutes. Untreated control cells were stained under the same conditions. The fluorescence signal was measured using a fluorescence microscope with FITC filter.
Flow cytometry of 2-NBDG uptake in CHO-K1 cells using the 2-NBDG Glucose Uptake Assay Kit. CHO-K1 cells were treated with or without 100 uM Phloretin at 37°C for 1 hour, then incubated with 100 uM 2-NBDG staining solution for 20 minutes. To prepare adherent CHO-K1 cells for flow cytometry, EDTA was used to detach cells after staining. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.