Pre-treatment is an essential step for effective FISH on Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections. This kit contains key reagents used to pretreat FFPE tissue sections from dewaxing with xylene through proteolytic digestion to helps permeabilization of cell membranes to facilitate penetration of fluorescence labeled probes. After proteolytic digestion, proceed the subsequent steps with the FISH assay protocol.
Supplied Product:
Kit content:1. 1x Paraffin Pretreatment Solution, 500 mL2. Protease (Pepsin), 0.5 mL x 10 vials
Storage Instruction:
After arrival, please store the Paraffin Pretreatment Solution at room temperature (RT), and store the Protease (Pepsin) at -20°C.
Protocol:
Protocol Download
MSDS:
Download
Regulation Status:
For research use only (RUO)
Datasheet:
Download
Publication Reference
1.
Deficiency of PTEN and CDKN2A Tumor-Suppressor Genes in Conventional and Chondroid Chordomas: Molecular Characteristics and Clinical Relevance.Chenlong Yang, Jianjun Sun, Lei Yong, Chen Liang, Tie Liu, Yulun Xu, Jun Yang, Xiaoguang Liu.Onco Targets Ther. 2020 May 25;13:4649-4663. doi: 10.2147/OTT.S252990. eCollection 2020.
2.
Clinical significance of PD-1/PD-Ls gene amplification and overexpression in patients with hepatocellular carcinoma.Ma LJ, Feng FL, Dong LQ, Zhang Z, Duan M, Liu LZ, Shi JY, Yang LX, Wang ZC, Zhang S, Ding ZB, Ke AW, Cao Y, Zhang XM, Zhou J, Fan J, Wang XY, Gao Q.Theranostics. 2018 Nov 10;8(20):5690-5702. doi: 10.7150/thno.28742. eCollection 2018.
3.
FGF19 genetic amplification as a potential therapeutic target in lung squamous cell carcinomas.Zhang X, Kong M, Zhang Z, Xu S, Yan F, Wei L, Zhou J.Thorac Cancer. 2017 Sep 14. [Epub ahead of print]
4.
Investigation of Molecular Alterations of AKT-3 in Triple Negative Breast Cancer.O"Hurley G, Daly E, O"Grady A, Cummins R, Quinn C, Flanagan L, Pierce A, Fan Y, Lynn MA, Rafferty M, Fitzgerald D, Pont幯 F, Duffy MJ, Jirstr闣 K, Kay EW, Gallagher WM.Histopathology. 2013 Oct 19. doi: 10.1111/his.12313.