One-Step ChIP Kit is use for investigating interactions of proteins and DNA.
Storage Instruction:
Store CH1, Non-Immune IgG, Anti-RNA Polymerase II, Proteinase K, GAPDH Primer-Forward, GAPDH Primer-Reverse, and 8-Well Assay Strips (With 1 Frame) at 4°C away from light. Store all other components at room temperature away from light.
Note:
Result Data
The enrichment of RNA polymerase II in GAPDH and MLH1 promoters with chromatin extract from formadehyde fixed colone cancer cells.
Protocol:
Protocol Download
MSDS:
Download
Suitable Sample:
Chromatin Extract
Sample Volume:
The amount of chromatin for each reaction can be 0.1 ug (about 1 x 104cells) to 15 ug (about 1.5 x 106cells). For an optimal reaction, the input chromatin amount should be 5 to 10 ug (about 0.5 to 1 x 106cells), as enrichment of target proteins to genome loci varies and some of the target proteins are of low abundance.