8-hydroxy-2"-deoxyguanosine ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of 8-hydroxy-2"-deoxyguanosine (8-OHdG) in tissue, serum, plasma and urine resulting from oxidative damage to DNA.
Calibration Range:
0.5 to 200 ng/mL
Quality Control Testing:
Standard curveThe standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction:
Store the kit at 4°C in the dark.
Protocol:
Protocol Download
MSDS:
Download
Suitable Sample:
Plasma, Serum, Tissue, Urine
Sample Volume:
50 uL
Label:
HRP-conjugate
Detection Method:
Colorimetric
Regulation Status:
For research use only (RUO)
Datasheet:
Download
Publication Reference
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Hypothermic oxygenated perfusion protects from mitochondrial injury before liver transplantation.Andrea Schlegel, Xavier Muller, Matteo Mueller, Anna Stepanova, Philipp Kron, Olivier de Rougemont, Paolo Muiesan, Pierre-Alain Clavien, Alexander Galkin, David Meierhofer, Philipp Dutkowski.EBioMedicine. 2020 Sep 23;60:103014. doi: 10.1016/j.ebiom.2020.103014.
2.
Ethanolic Extract of Moringa oleifera Leaves Influences NF-κB Signaling Pathway to Restore Kidney Tissue From Cobalt-Mediated Oxidative Injury and Inflammation in Rats.Mohamed M Abdel-Daim, Samah R Khalil, Ashraf Awad, Ehsan H Abu Zeid, Reda Abd El-Aziz, Hamed A El-Serehy.Nutrients. 2020 Apr 9;12(4):1031. doi: 10.3390/nu12041031.
3.
Protective potency of Astragalus polysaccharides against tilmicosin- induced cardiac injury via targeting oxidative stress and cell apoptosis-encoding pathways in rat.Ashraf Awad, Samah R Khalil, Basma M Hendam, Reda M Abd El-Aziz, Mohamed M M Metwally, Tamer S Imam.Environ Sci Pollut Res Int. 2020 Jun;27(17):20861-20875. doi: 10.1007/s11356-020-08565-y. Epub 2020 Apr 4.
4.
An integrated perfusion machine preserves injured human livers for 1 week.Eshmuminov D, Becker D, Bautista Borrego L, Hefti M, Schuler MJ, Hagedorn C, Muller X, Mueller M, Onder C, Graf R, Weber A, Dutkowski P, Rudolf von Rohr P, Clavien PA.Nat Biotechnol. 2020 Feb;38(2):189-198. doi: 10.1038/s41587-019-0374-x. Epub 2020 Jan 13.
5.
Restoring strategy of ethanolic extract of Moringa oleifera leaves against Tilmicosin-induced cardiac injury in rats: Targeting cell apoptosis-mediated pathways.Khalil SR, Abdel-Motal SM, Abd-Elsalam M, Abd El-Hameed NE, Awad A.Gene. 2020 Mar 10;730:144272. doi: 10.1016/j.gene.2019.144272. Epub 2019 Dec 5.
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Differential susceptibility of kidneys and livers to proliferative processes and transcriptional level of the genes encoding desmin, vimentin, connexin 43, and nestin in rats exposed to furan.Awad A, Khalil SR, Farag MR, Nassan MA.Ecotoxicol Environ Saf. 2018 Jul 6;162:235-244.
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Spermatozoal Fractalkine Signaling Pathway Is Upregulated in Subclinical Varicocele Patients with Normal Seminogram and Low-Level Leucospermia.Abo El-Khair SM, Gaballah MA, Abdel-Gawad MM, Ismail SRM, Elsamanoudy AZ.Adv Urol. 2017 Dec 21;2017:5674237.
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Role of betaine in liver injury induced by the exposure to ionizing radiation.Shedid SM, Abdel-Magied N, Saada HN.Environ Toxicol. 2018 Oct 11. doi: 10.1002/tox.22664. [Epub ahead of print]
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The antifibrotic and fibrolytic properties of date fruit extract via modulation of genotoxicity, tissue-inhibitor of metalloproteinases and nuclear factor- kappa B pathway in a rat model of hepatotoxicity.Attia H, Al-Rasheed N, Mohamad R, Al-Rasheed N, Al-Amin M.BMC Complement Altern Med. 2016 Oct 24;16(1):414.
10.
Warm vs. cold perfusion technique to rescue rodent liver grafts.Schlegel A, Kron P, Graf R, Dutkowski P, Clavien PAJ Hepatol. 2014 Jul 30. pii: S0168-8278(14)00522-4. doi: 10.1016/j.jhep.2014.07.023.
11.
Hypothermic oxygenated perfusion (HOPE) protects from biliary injury in a rodent model of DCD liver transplantation.Schlegel A, Graf R, Clavien PA, Dutkowski PJ Hepatol. 2013 Jun 29. pii: S0168-8278(13)00439-X. doi: 10.1016/j.jhep.2013.06.022.
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Carnosine sustains the retention of cell morphology in continuous fibroblast culture subjected to nutritional insult.Kantha SS, Wada S, Tanaka H, Takeuchi M, Watabe S, Ochi H.Biochem Biophys Res Commun. 1996 Jun 14;223(2):278-82.
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Oxidative damage to DNA during aging: 8-hydroxy-2"-deoxyguanosine in rat organ DNA and urine.Fraga CG, Shigenaga MK, Park JW, Degan P, Ames BN.Proc Natl Acad Sci U S A. 1990 Jun;87(12):4533-7.
14.
Formation of 8-hydroxyguanine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair.Kasai H, Crain PF, Kuchino Y, Nishimura S, Ootsuyama A, Tanooka H.Carcinogenesis. 1986 Nov;7(11):1849-51.