| | CatalogNo.: K-VBlue
Description: VitaBlueisaproprietaryfluorometric/colorimetricgrowthindicatorbasedondetectionofmetabolicactivityofcellls. Itisnon-toxictocellsandmeasurestheoxidation-reduction(redox)environmentinthemedium.ViablecellsreduceVitaBluefromitsoxidizedform(nonfluorescent,bluecolor)tothereducedform(fluorescent,redcolor)duringcellgrowth. ThefluorescenceintensityofVitaBlueat540ex/590emis proportionaltothenumberofviablecellsinthesample.Ifafluorescenceplatereaderis unavailable,absorbancedifferenceat570nmand600nmcanbeusedinstead. Pleaseseegraphsbelowforexample. VitaBlueissuitableforvarioushuman/animalandplantcelllines,bacteria,mycobacteria,andfungi. ItcanbeusedtoassesscellviABIlity,cellproliferation,andcytotoxicity.Itisnontoxic,simpletouse,sensitive,stable,andhighlyreproducIBLe. | |
Storage- Lightsensitive,storetightlycappedinthedarkat4-22°Cfor12months.VitaBluecanbestoredat-20°Ctoextendtheshelflife.Ifstoredfrozen,ensureallcomponentsareinsolutionbywarmingto37°Candmixingasnecessarybeforeuse.
SuggestedUse- VitaBlueisagreattoolincancerresearch,cellviabilitystudy,proliferationstudy,drugcytotoxicitystudy,andcellgrowthrelateddrug/inhibitor/stimulatorscreens.
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