ClassIPhosphoinositide3-kinases(PI3-K),areubiquitouslyexpressedlipidkinaseswhichphosphorylatePI(4,5)P2atthe3'-hydroxyloftheinositolringproducingPtdIns(3,4,5)P3.PtdIns(3,4,5)P3hasshowntobeanimportantlipidsecondmessengerwithkeyrolesinfundamentalcellularresponsessuchasproliferationandsurvival.DuetotheseinterestingBIOLOGicalroles,PI3-KandPtdIns(3,4,5)P3havebecomeattractivetargetsforresearchanddrugdevelopmentinmanydiseasesincludinginflammationandcancer.EchelonhasmultipleformatsavailableforthedeterminationofclassIPI3-Kactivity. |