HyaluronidasesareagroupofenzymesthatdegradeHyaluronan(HA),alinearpolysaccharidecomprisedofarepeatingdisaccharideofN-acetylglucosamineandD-glucuronicacid.HAisinvolvedinmanybiologicalprocessesincludingstructuralsupport,cellmigrationandtissueturnover.CirculatingHAlevelshavealsobeenshowntocorrelateinseveraldisorderssuchasliverdiseaseandcertaintypesofcancer.HyaluronidaseshavebecomeanimportantareaofstudyduetotheirregulatoryfunctioninHAmetabolismandtheirroleinphysiologicalprocessessuchasfertilizationandwoundhealing.ElevatedexpressionlevelsofthegeneencodingHyaluronidase1havebeenfoundinatypicalductalhyperplastictissues,suggestingthepossibilitythatitmaybeaviablebioMarkerforidentifyingpremalignantlesionsthatcandevelopintobreastcancer.Researchhasalsoshownthathyaluronidasesareinvolvedinseveralpathologicalprocessessuchasbacterialpathogenesis,thespreADIngoftoxins/venomsandcancerprogression,makinghyaluronidasesapotentialpharmacologicaltarget.
Echelon’sHyaluronidaseActivityELISAisaquantitativeimmunoassaydesignedforinvitromeasurementofhyaluronidaseactivityinbiologicalsamples.Hyaluronidasereactionsareperformedina96-wellmicrotiterplatepre-coatedwithHAsubstrate.TheactivityofthehyaluronidaseisdeterminedbycomparingHAsubstratelevelspostreactiontoastandardcurveofpre-coatedHAsubstrateamounts.Echelon’sHyaluronidaseActivityELISAhasbeenvalidatedwithhumansera,plasmaandurinesamplesinadditiontopurifiedhyaluronidasefrombovinetestes.Echelon’sHyaluronidaseActivityELISAprovidesarobustandsimplemethodforresearcherstomeasurehyaluronidaseactivityinbiologicalsamples. StoragePart1at-20°CandPart2at4°C
RelatedProducts:HAELISA(K-1200),HASandwichELISA(K-4800),CollagenIVELISAKit(K-1700).
ProductKeywords:Hyaluronan,Hyaluronate,LiverCirrhosis,RheumatoidArthritis,Osteoarthritis,LiverFibrosis
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