ProductBackground:Autotaxin,alsoknownasATX,ENPP2,lysophospholipaseD(lysoPLD),phosphodiesterase1αandplasmacellglycoprotein-1,isasecretedglycoproteinthatiswidelyexpressedwithhighlevelsintheserum.ViaitslysoPLDactivity,autotaxinhydrolyzeslysophosphatidylcholine(LPC)togeneratethephospholipidgrowthfactorlysophosphatidicacid(LPA).Theenzyme’ssameactivityhydrolyzessphingosylphosphorylcholine(SPC)toformsphinosine-1-phosphate(S1P).Autotaxinwasfirstisolatedastheautocrinemotilityfactorsecretedfrommelanomacells.Atthattime,theenzyme’slysoPLDactivityhadnotbeenidentified,soitwasclassifiedbyhomologytotheecto-nucleotidepyrophosphatase/phosphodiesterase(NPP)familyofenzymeswhosemembershydrolyzephosphodiesterbondsinvariousnucleotidesandnucleotidederivatives.Itwasinitiallyunclearhownucleotidehydrolysiscouldleadtothestimulationofcellmotility.ThismysterywassolvedwhenautotaxinwasdiscoveredtobeidenticaltoserumlysoPLD.Sincethen,thecancer-relatedactivitiesofautotaxin,atleastinculturedcells,havebeenattributedtotheenzyme’slysoPLDactivity.Inadditiontocancer,autotaxinhasbeenimplicatedinanumberofdiseasesincludingobesity,arthritis,multiplesclerosis,Alzheimer’sdiseaseandneuropathicpain. Echelon’sATXSandwichELISAprovidesarobustandsimplemethodforresearcherstomeasureATXinbiologicalsamples.TheconcentrationofATXinthesampleisdeterminedusingastandardcurveofknownamountsofATX.Theassaywasvalidatedwithsera,plasma,urineandovarianascites.
RelatedProducts: AutotaxinActivityAssay(K-4100) AutotaxinInhibitorScreeningKits:96-well(K-4200)and384-well(K-4200HTS) AutotaxinSubstrate:FS-3(L-2000) AutotaxinInhibitors:HA-130(B-0701),BrP-LPA(L-7416)andThio-ccPA(L-7118) AutotaxinEnzyme,active(E-4000)
ProductKeywords:Autotaxin,ATX,LPA,LPC,lysophospholipaseD
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