INPP4Aisamagnesium-independentphosphataseinvolvedinthephosphatidylinositol3-kinase(PI3K)pathway.INPP4AanditsisoenzymeINPP4Bhydrolyzethe4-phosphatefromthelipidsecondmessengerphosphatidylinositol-3,4-bisphosphate(PI(3,4)P2)toformphosphatidylinositol-3-phosphate(PI(3)P).Whilelittleevidencesupportsitsroleasatumorsuppressor,INPP4AsuppressesglutamateexcitotoxicityinthecentralnervoussystemandisresponsIBLefortheWeeblemousephenotype.AspontaneousframeshiftmutationintheInpp4ageneispresentintheWeeblemouseresultinginselectiveneuronallossinthecerebellumresulting,severeataxiaandearlydeath. TheINPP4ELISAisacompetitiveassayinwhichthesignalisinverselyproportionaltotheamountofPI(3)Pproduced.AftertheINPP4reactionsarecomplete,reactionproductsareaddedtothePI(3)P-coatedmicroplateforcompetitivebindingtoaPI(3)Pdetectorprotein.TheamountofPI(3)Pdetectorproteinboundtotheplateisdeterminedthroughcolorimetricdetection.Theassaydisplaysexcellentrobustness(Z’value~0.8). Storage:Part1at4°C,Part2at-20°CRelatedProducts:INPP4A,active(E-8000) INPP4substrates:diC8-PI(3,4)P2(P-3408),diC16-PI(3,4)P2(P-3416) MalachiteGreenPhosphataseAssayKits |