ProductBackground:TheassaydevelopedbyEchelonBiosciencesInc.usesthefluorogenicautotaxinsubstrateFS-3.FS-3isanLPCanaloguethatisconjugatedwithbothafluorophoreandaquencher.Initsnativestatethequencherinterfereswiththefluorophore’sfluorescence.OnceautotaxincleavesFS-3,thefluorophorebecomesliberatedfromthequencher,resultinginincreasedfluorescence. Thisassaygivesaquantitativemeasurementofautotaxinactivityinbiologicalsamplesbymeasuringchangesinfluorescenceovertime.Becausetheassayisnotendpointbased,ithastheadvantageofbeinglesssusceptIBLetoerrorarisingfrommistimingtheadditionofcomponentstodifferentsamplewells.Thefinalreadoutisinquantitativeunitsthatcorrectforinconsistenciesbetweendifferentusersequipment. FeaturedinPublications:1)Shelton,E.L.,C.L.Galindo,etal.(2013)."AutotaxinSignalingGovernsPhenotypicHeterogeneityinVisceralandParietalMesothelia."PLoSONE8(7):e69712. 2)Gaetano,C.G.,N.SamADI,etal.(2009)."Inhibitionofautotaxinproductionoractivityblockslysophosphatidylcholine-inducedmigrationofhumanbreastcancerandmelanomacells."MolecularCarcinogenesis48(9):801. 3)Braeuer,R.R.,M.Zigler,etal.(2012)."Galectin-3ContributestoMelanomaGrowthandMetastasisviaRegulationofNFAT1andAutotaxin."CancerResearch72(22):5757-5766.
RelatedProducts: AutotaxinInhibitorScreeningKits:96-well(K-4200)and384-well(K-4200HTS) AutotaxinSubstrate:FS-3(L-2000) AutotaxinInhibitors:BrP-LPA(L-7416),HA130(B-0701),PF8380(B-0702),andThio-ccPA(L-7118) AutotaxinEnzyme,active(E-4000)
NoticetoPurchaser:EchelonBiosciencesproductsaresoldforresearchanddevelopmentpurposesonlyandarenottobeincorporatedintoproductsforresalewithoutwrittenpermissionfromEchelonBiosciences.ThecompoundFS-3anditsuseinassayingforLysophospholipaseDactivityarecoveredbyEchelonBiosciencesInc.USPatent7,989,663.Thepurchaseofthisproductincludesalimited,non-transferableimmunityfromsuitundertheforegoingpatentclaimsforusingonlythisamountofproductforthepurchaser'sowninternalresearch.
ProductKeywords:ATX,LPA,FS-3,LPC,lysophospholipaseD |