TheassayisacompetitiveELISAwherethesignalisinverselyproportionaltotheamountofPI(3,4)P2produced.ThisallowstheusertomeasuretheamountofPI(3,4)P2extractedfromcellsbymeansofstandard96wellELISAformat,eliminatingtheneedforrADIoactivityandthinlayerchromatography.OncePI(3,4)P2hasbeenextractedfromcellularsamples,itisincubatedwithaPI(3,4)P2detector,thenaddedtothePI(3,4)P2-coatedplateforcompetitivebinding.Aperoxidase-linkedsecondarydetectionreagentandcolorimetricsubstrateisusedtodetectPI(3,4)P2detectorbindingtotheplate.ThecolorimetricsignalisinverselyproportionaltotheamountofPI(3,4)P2extractedfromcells. Format:96-wellCompetitiveELISAStorage:2-8°CRelatedProducts:PIPMassAssaysReferences:1)NonradioactiveQuantificationofPhosphoinositide3-kinase(PI3K)ProductsfromBreastCancerCellLines 2.QuantificationofPI3-KinaseProductsfromBreastCancerCellLines
Keywords:PI(3,4)P2,PI34P2,PtdIns(3,4)P2 |