Echelon/Sphingosine 1-Phosphate ELISA Kit/EA KIT/K-1900

The Sphingosine 1-Phosphate ELISA kit (S1P ELISA) is a sensitive and robust method for the quantification of S1P in biological samples (96-well format).

Assay Range: 0.0625 µM – 2 µMSample Volume: For duplicate data points 25 µL of each sample is required.Sample Type: This kit detects S1P from human or animal in serum, plasma, tissue homogenate, and cell lysate, This assay is not species specific and has been tested with S1P from human, bovine, equine, caprine, and mouse.

Product BackgroundThe Sphingosine 1-Phosphate ELISA kit (S1P ELISA) measures S1P in biological samples.

To run the S1P ELISA, the S1P coated plate is first blocked to reduce non-specific binding. Then the S1P standards and samples are combined with an anti-S1P antibody before it is transferred to the blocked S1P coated plate. The  S1P coated plate and the S1P in the sample compete for binding of the anti-S1P antibody during this step. Following an incubation and plate wash, streptavidin-HRP is added to the plate for detection of any bound anti-S1P antibodies (labeled with biotin). After an additional incubation and plate wash, TMB substrate is added to the plate. The colorometric TMB reaction is stopped with the addition of 1N sulfuric acid and the plate is measured at 450 nm absorbance. The concentration of S1P, in the samples, is determined when compared to the S1P standard curve.

Sphingosine 1-Phosphate (S1P) is a key component of the sphingolipid signaling cascade. S1P initiates a proliferative, pro-angiogenic and anti-apoptotic sequence of events contributing to cancer progression. Recently, scientific literature has suggested that S1P is a potent tumorigenic growth factor that is likely released from tumor cells and that S1P may be a novel biomarker for early stage cancer detection. Sphingosine kinase has also been shown to be up-regulated in a variety of cancer types (S1P is produced via the activity of sphingosine kinase phosphorylating sphingosine).

Detection of S1P Lyase activity is also possible with this assay using a modified protocol. This protocol can be found on the documents tab.

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Notice to Purchaser: Echelon Biosciences products are sold for research and development purposes only and are not to be incorporated into products for resale without written permission from Echelon Biosciences. This kit is covered by a U.S. Patent and includes a limited, non-transferable license under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. For inquiries email echelon@echelon-inc.com.