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 | CatalogNo.: K-1000s Description: Echelon'sPI3-KinaseActivityELISA:Picoisacompetitive(96-well)ELISAassaykitthatmeasuresClassIPhosphoinositide3-kinases(PI3-K)activitydirectlythroughitsreactionproduct,PtdIns(3,4,5)P3(PIP3).ThisassaycanbeusedforinvitrodeterminationofPI3-Kactivityincancertissuesandcelllinesorcompoundscreeningfordrugdiscovery Assaysensitivity:Thisassaycanmeasureaslowas0.22pmolPIP3anddetectactivityfromaslittleas31ng/mLPI3-Kenzyme AssayIncubationtime:2.5hr SampleType:Purifiedand/orImmunoprecipitated(IP)PI3-KEnzyme(seeIPprotocolattheendoftheK-1000sTDS) | ||
ProductBackground:PI3-KreactionsarerunwiththeClassIPI3-KphysiologicalsubstratePI(4,5)P2(PIP2).Theenzymereactions,PIP3standards,andcontrolsarethenmixedandincubatedwithPIP3bindingproteinthatishighlyspecificandsensitivetoPIP3.ThismixtureisthentransferredtoaPIP3-coatedmicroplateforcompetitivebinding.Afterwards,aperoxidase-linkedsecondarydetectorandcolorimetricdetectionisusedtodetecttheamountofPIP3producedbyPI3-KthroughcomparingtheenzymereactionswithaPIP3standardcurve. FeaturedinPublications:1.A.E.Awad,V.Kandalam,S.Chakrabarti,X.Wang,J.M.Penninger,S.T.Davidge,G.Y.OuditandZ.Kassiri.(2010)"TumornecrosisfactorinducesmatrixmetalloproteinasesincardiomyocytesandcardiofibroblastsdifferentiallyviasuperoxideproductioninaPI3K?-dependentmanner"AmJPhysiolCellPhysiol298(3),C679-C6922.B.Venkatesan,S.D.Prabhu,K.Venkatachalam,S.Mummidi,A.J.Valente,R.A.Clark,P.DelafontaineandB.Chandrasekar.(2010)"WNT1-inducIBLesignalingpathwayprotein-1activatesdiversecellsurvivalpathwaysandblocksdoxorubicin-inducedcardiomyocytedeath"CellSignal22(5),809-20 3.J.J.Wallin,J.Guan,K.A.etal.(2012)"ActivePI3KPathwayCausesanInvasivePhenotypeWhichCanBeReversedorPromotedbyBlockingthePathwayatDivergentNodes"PLoSOne7(5),e36402 4.C.M.Furtado,M.C.Marcondes,R.S.Carvalho,M.Sola-PennaandP.Zancan.(2015)"Phosphatidylinositol-3-kinaseasaputativetargetforanticanceractionofclotrimazole"TheInternationalJournalofBiochemistry&CellBiology62,132-141 5.Poopal,A.C.,L.M.Schroeder,etal.(2016).IncreasedexpressionofthePI3Kcatalyticsubunitp110?underlieselevatedS6phosphorylationandproteinsynthesisinanindividualwithautismfromamultiplexfamily.7:1-12.doi:10.1186/s13229-015-0066-4 RelatedProducts: ClassIPI3KActivityAssays:Echelonhasmultipleformatsavailable.PleaseseethePI3-KAssayComparisonChart formoreinformation. ClassIIIPI3KActivityAssay(K-3000) LipidMassELISA's:PIP3MassELISA(K-2500s)andPI(4,5)P2MassELISA(K-4500) HumanPI3KEnzymes:alpha(p110α/p85α),beta(p110β/p85α),PI3KαE545K–ActiveMutant(p110α/p85α) MousePI3KEnzyme:PI3K(p110α/p85α) PI3KInhibitors NoticetoPurchaser:EchelonBiosciencesproductsaresoldforresearchanddevelopmentpurposesonlyandarenottobeincorporatedintoproductsforresalewithoutwrittenpermissionfromEchelonBiosciences.Thiskitandallnon-rADIoactive,competitiveassaysfordeterminingphosphoinositide-3-kinase(PI3-K)activityarecoveredbyEchelonBiosciencesInc.U.S.Patent7,067,269.Thepurchaseofthisproductincludesalimited,non-transferableimmunityfromsuitundertheforegoingpatentclaimsforusingonlythisamountofproductforthepurchaser'sowninternalresearch. ProductKeywords:PI3-Kinase,PI3K,Phosphatidylinositol3-kinases,PI3-kinases,PI3Ks,K-1000 BulkDiscounts: | |||
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