ApplicationNotes | AntibodyispredilutedandreadytouseforImmunohistochemistryofformalin-fixed,paraffin-embeddedtissues.
Pretreatment:HeatInducedEpitopeRetrieval(HIER).RecommendEDTA,pH8.0(Cat.No.21546).NosignalwasdetectedwithoutEpitoperetrieval.
Incubation:10minuteswithIHCSelect®DetectionKits.
CD4hasbeenpredilutedforuseastheprimaryantibodywithChemicon"sIHCSelect®DetectionKitsandProtocols(CatalogNos.DAB050,DET-HP1000,APR050,andDET-APR1000),butothersupplier"sIHCdetectionsystemsmaybeused.Foroptimizedprotocoldetails,visitwww.chemicon.comandselecttheprotocolslinkunderCat.No.IHCR2110-6. |