Description
Image-iT®FXsignalenhancerisaliquidthatisapplieddirectlytoslidesorcoverslipscontainingfixedandperme
ABIlizedcellortissuesamplespriortostainingwithfluorescentprobes.WhenImage-iT®FXsignalenhancerisused,nonspecificfluorescence(background)commonlyseenwiththeapplicationoffluorescentconjugatesofstreptavidin,goatanti-mouse,orgoatanti-rabbitIgGislargelyeliminated.
KeyAttributes•
Easyprotocol—applyenhancerfromready-madesolutionindropperbottle•
Effectivewithmanyfluorescentlabels[seeTable2intheproductmanual]•
Costeffective—suppliesenoughfor50standardcoverslips•
CompatIBLewithyourimmunostainingprotocol’sotherblockingstepsReduceNonspecificBindingofDyestoTissues.AlexaFluor®dyesandmanyotherdyeshavenegativelychargedmodificationsthatcanleadtononspecificassociationwithcellsandtissue.Whilenotalwayssignificant,itcanbeaproblemwhenpushingthelimitsofsensitivityinlow-abundancetargets.Image-iT®FXsignalenhancercansignificantlyreducethisbinding,resultinginmarkedimprovementsinstaining.
Needhelpdecidingwhatproductyouneedforyourcellimaging?Seeourcellimagingworkflowanddecisiontree.
Tiredofpoorsignal-to-noise?Seeourcollectionantifadeandsignalenhancerproductsfrom
Molecularprobes®.
ForResearchUseOnly.Notintendedforanyanimalorhumantherapeuticordiagnosticuse.
ForResearchUseOnly.Notforuseindiagnosticprocedures.